Coding

Part:BBa_K3788006:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)


ConA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal XhoI site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 406
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.


Source

The ConA sequence was taken from uniprot sequence of ConA and liker was created by ourself

References